Influence of PTHR1 and COL1A1 genes polymorphism on the development of periodontal tissue diseases in young people

M. A. Luchynskyi, Y. V. Boliuk, V. M. Luchynskyi

Abstract


At the present stage of development of dentistry, the leading Ukrainian and foreign scientists devote a considerable part of the research to a deeper study of the etiology and pathogenetic mechanisms of periodontal tissue diseases and the influence of various exogenous and endogenous factors on their course.

The aim of the study – to learn the ability and methods of forecasting and early diagnosis of the periodontal tissue lesions in young people.

Materials and Methods. During our research we examined 24 young people with periodontal tissue diseases, who were included to the main group, and 15 healthy people, who formed the control group. The complex clinical examination was performed in each research group. It was studied the distribution of polymorphous variants of the type I parathormone receptor and the α1-chain of collagen gene with a help of polymerase chain reaction by restrictase cleavage of DNA fragments and electrophoresis in polyacrylamide gel (AA/BA 29:1).

Results and Discussion. The distribution of genotypes by PTHR1 gene in control group was similar to those in main group (p>0.05). Also we didn’t find the difference between frequencies of the separate alleles in people with periodontal tissue pathology and without it (p>0.05). Yes, the repetitions of the allele 5 encoding normal type I parathormone receptor were found more often, comparing with the allele 6 that is responsible for the formation of unfunctional PTHR1 (р<0.001) in both main and control groups. The dominance of the genotype TT, which corresponds to the pathology, was found in young people with the periodontal tissue lesions – (38.46 ± 4.79) %, while among the control group the genotype of norm GG was met the most often – (68.24±5.08) %. Also, the frequency of repetitions of the allele T encoding the imperfect collagen chain was (57.60±3.79) % in young people with periodontal diseases, and in the control group this figure was (13.27±2.81) %, p<0.001. 

Conclusions. According to our results the presence of allele T and genotype TT that correspond the imperfect collagen chain may be one of the causes of periodontal tissue pathology.


Keywords


periodontium; genes; bone tissue; young people.

References


Averianov, S.V., & Zubareva, A.V. (2015). Vzaimosvyaz mezhdu zubochelyustnymi anomaliyami i zabolevaniyami parodonta [Connection between dentoalveolar abnormalities and periodontal diseases]. Problemy stomatologii – Problems in Dentistry, (2), 46-48 [in Russian].

Katkhanova, L.S., Akulova, E.V., Lysov, A.V., Mogila, A.P. (2014). Vliyanie gormonalnoy regulyatsyy na sostoyanie tkaney parodonta [Influence of the hormone regulation on the periodontal tissues]. Bulletin of Medical Internet Conferences, 4 (12), 1342 [in Russian].

Krylov, M.Yu., Myakotkin, V.A., & Benevolenskaya, L.I. (2004). Analiz polimorfizmov genov shchelochnoy fosfatazy, retseptora lipoproteina nizkoy plotnosti i vitamin D-svyazyvayushchego belka kak vozmozhnykh genov-kandidatov, uchastvuyushchykh v determinatsii mineralnoy plotnosti kosti u zhenshchin v postmenopauze s pervichnym osteoporozom [Analysis of alkaline phosphatase, soluble acid phosphatase, low density lipoprotein and vitamin D binding protein gene polymorphisms as possible gene-candidates participating in mineral bone density determination in women with primary osteoporosis in postmenopause]. Nauchno-praktycheskaya revmatoloiya – Scientifical and Practical Rheumatology, (3), 10-14 [in Russian].

Luchynskyi, М.А., Luchynskyi, V.M., Petruniv, V.B., & Luchynska, Yu.I. (2012). Imunolohichna reaktyvnist orhanizmu itei yaki prozhyvaiut v zoni vplyvu nehatyvnykh chynnykiv dovkillia [Immunological reactivity of the organism of children living in the zone of influence of negative environmental factors]. Visnyk sotsialnoi hihiieny ta orhanizatsii okhorony zdorovia Ukrainy – Journal of Social Hygiene and Organizations of Public Health of Ukraine, 3 (55), 17-20 [in Ukrainian].

Orekhova, L.Yu., Neyzberg, D.M., Styuf, I.Yu. (2006). Kliniko-immunologicheskie i mikrobiologicheskie paralleli pri khronicheskom generalizovannom parodontite i yazvennoy bolezni zheludka [Clinical, immunological and microbiological aspects of chronic generalized periodontitis and ulcer disease]. Stomatologiya – Dentistry, (6), 22-26 [in Russian].

Safonova, A.V. (2012). Kliniko-geneticheskie prediktory razvitiya zabolevaniy parodonta [Clinical and genetic predictors of periodontal diseases development]. Moscow [in Russian].

Tsepov, L.M., Nikolaev, A.I., & Mikheeva, E.A. (2004). Faktory agressii i faktory zashchity v patologii parodonta vospalitelnogo kharaktera [Aggressive and protective factors of periodontal pathology with inflammatory genesis]. Parodontologiya – Periodontology, 1 (30), 3-7 [in Russian].

Zinoveva, A.I, Atrushkevich, V.G., Polyakov, A.V., & Shagina, O.A. (2011). Geneticheskie paralleli v multifaktornykh modeliakh parodontita s agressivnym techeniem i osteoporoza [Genetic aspects of aggressive periodontitis multifactor models and osteoporosis]. Rossiyskaya stomatologiya – Russian Dentistry, (6), 34-40 [in Russian].

Hasiuk, N.V., Yeroshenko, H.A., & Palii, O.V. (2013). Suchasni uiavlennia pro etiolohiiu ta patohenez khvorob parodonta [Modern views on etiology and pathogenesis of periodontal diseases]. Svit medytsyny ta biolohii – World of Medicine and Biology, (2), 207-211 [in Ukrainian].

Atrushkevich, V.G. (2010). Diagnostika i lechenie zabolevaniy parodonta pri narushenii mineralnogo obmena [Diagnosis and treatment of periodontal diseases in case of mineral metabolism disorders]. Moscow [in Russian].

Brin, V.B., Vartapyan, I.A., & Daniyarov, S.B. (1994). Osnovy fiziologii cheloveka: uchebnik dlya vysshykh uchebnykh zavedeniy: v 2 t., pod redaktsyei akad. RAMN B.I. Tkachenko [Bases of human physiology: textbook for higher educational institutions: in 2 vol., under ed. acad. B.I. Tkachenko]. Saint-Petersburg [in Russian].




DOI: http://dx.doi.org/10.11603/2311-9624.2017.2.7936

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