MORPHOLOGICAL CHANGES IN LUNGS, HEART AND LIVER CAUSED BY EXPERIMENTAL ASSOCIATED CHEST AND THIGHS TRAUMA

M. M. Khudobiak, M. I. Marushchak, L. M. Holovatiuk, T. I. Datsko

Abstract


Background. According to preliminary results obtained, the development of hypoxemia caused by chest trauma affected lipid peroxidation stimulation leading to disruption of cell membranes structure and consequently cell death closing the ‘vicious’ circle, which in our opinion and according to the literature cause multiple organ failure.

Objective. The aim of our study was to determine the features of morphological changes in cases of heart, lungs, liver injury after associated chest and both thighs trauma in rats.

Methods. For histological examination the pieces of left ventricle tissues of heart, lungs and liver were used, which were fixed in 10% neutral formalin solution, dehydrated and embedded in paraffin blocks.

Results. It was established that associated trauma of chest and both thighs in 1 day after the injury is accompanied by disturbances of hemodynamics in parenchymal organs due to venous congestion that occurs during the development of cardiovascular disease caused by traumatic shock. In the most severe period of maximum likelihood of complications, within 3 days, respiratory hypoxia, cardiovascular disorders, metabolic acidosis, and induced programmed cell death may develop.

Conclusions. On the background of increasing pulmonary oedema (thickening of inter air sac membranes) caused by associated trauma of chest and both thighs in rats after only one day of the experiment hemodynamic changes in heart (low-blood supply of vessels in epicardium and isolated blood vessels of venous type) and liver (mainly plethora of blood vessels) were observed, which lead to morphological changes of myocardium within 3 days.


Keywords


trauma; lungs; liver; heart; morphological changes.

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References


Schedrenok VV, Yakovenko SЕ, Mohuchaya OV. Clinical and organizational aspects of combined craniocerebral injuries. S-Petersburg. 2010;435.

Humanenko EK. Polytrauma. Actual problems and new technology in treatment. Materials of international conference: New technologies in the military and peace time surgery. 2006 April 20-22. S-Petersburg. 2006;4–14.

Gayko GV, Kalashnikov AV, Lymar EO. State and problems of orthopedic and trauma care in Ukraine. Orthopedics, traumatology and prosthetics. 2004;2:5–9.

Gelfand BR. Integral systems for assessing the severity of patients with polytrauma. Bulletin of intensive care. 2004;1:58–65.

Berezka NI, Litovchenko VA, Garyachyy EB, et al. Optimization of surgical tactics for treating victims with polytrauma using the severity and damage severity scales. Scientific statements Bel GU. Medicine. Pharmacy. 2014;25(4):116-119.

Guriev CO, Tanasienko PV, Satsyk SP. Clinical and epidemiological characteristics of patients with infectious complications of trauma due to accidents. Medicine today and tomorrow. 2012;1:54.

Wang HE, Shapiro NI, Yealy DM. Characteristics of out-of-hospital shock care. Critical Care Medicine. 2011;39:243–245.

Pasternak VN. Features of biochemical homeostasis in people with severe pelvic trauma of the pelvis with an unfavorable course of traumatic illness. Archive of clinical and experimental medicine. 1997;6(30):8–15.

Pasternak VN, Khudobin VYu, Pasternak VV, Shpachenko NN, Chirach SH. Actual problems of the organization of assistance to victims in road traffic multiple and combined trauma. Orthopedics, traumatology and prosthetics. 2002;3:82–87.

Pridruga SM, Gasyuk NV. Nosotropic mechanisms of organs damage in different periods of traumatic illness. The world of medicine and biology. 2012;2:194–200.

Kozak DV, Volkov KS. Structural changes of some internal organs in animals with simulated polytrauma and correction with carbacetam. Scientific Bulletin of the Uzhgorod University, series "Medicine". 2014;2(50):3–6.

Marushchak MI, Khudobiak MM, Krynytska IYa, Antonyshyn IV. Lipid peroxidation in multiple organ failure caused by associated chest and hip trauma International Journal of Medicine and Medical Research. 2016;2(2):52–55.

Kozak DV, inventor; Ternopil State Medical University, the patent owner. Modeling method of polytrauma. Ukraine patent number 63997. 25 Oct 2011.

Goralska LP, Khomich BT, Kononsky OI. Histological techniques and methods of morphological studies in normal and pathological conditions. Zhytomyr: Polissia; 2011;288.

Malone DL, Kuhls D, Napolitano LM, et al. Back to basics: validation of the admission systemic inflammatory response syndrome score in predicting outcome in trauma. J Trauma. 2001;51:458–463.

Rotstein OD. Modeling the two-hit hypothesis for evaluating strategies to prevent organ injury after shock. Resuscitation. J Trauma. 2003;54(5):203–206.

Kalinkin CO, Kurapov EP, Kalinkin AO. Intensive therapy, energy and plastic provision of patients with polytrauma. The pain, anesthesia and intensive care. 2000;1:173–174.

Vologzhanin DA. Metabolic basis of secondary immune deficiency in traumatic disease: St Petersburg: 2005.

Eisner MD, Thompson BT, Schoenfeld D, et al. Acute Respiratory Distress Syndrome Network. Airway pressures and early barotraumas in patients with acute lung injury and acute respiratory distress syndrome. Am J Respir Crit Care Med. 2002;165(7):978–982.

Marik PE. Aspiration pneumonitis and aspiration pneumonia. N Engl J Med. 2001;344(9):665–671.




DOI: http://dx.doi.org/10.11603/ijmmr.2413-6077.2017.1.7625

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