AGE RELATED OXIDATIVE PROCESSES AND ENDOGENOUS INTOXICATION DYNAMICS OF RATS ARTER TOBACCO SMOKE AFFECTION

P. H. Lyhatskyy, O. B. Rytsyk, L. S. Fira, O. Z. Yaremchuk

Abstract


Background. In an experiment on rats in the content of reactive oxygen species and lipid peroxidation products in blood and liver of rats of different age groups after 45-day affection with tobacco smoke was studied.

Objective. The study was aimed to investigate the rate of reactive oxygen species formation, especially the processes of lipid peroxidation and degree of endogenous intoxication in rats of different age groups in terms of 45 day affection with tobacco smoke.

Methods. The content of ROS was determined in blood neutrophils method gradient centrifugation, the activity of free radical processes in rats evaluated the content of TBA - active products (TBA - AP), the degree of endogenous intoxication - the content average molecular weight (AMW) of the two factions – MW1 (dominated by chain amino acids) and MW2 (dominated by aromatic amino acids). Blood, blood serum and liver of the experimental rats were used for the investigation. It was prepared 10% homogenate in saline from liver tissue.

Results. We have noticed that the destruction of rats by tobacco smoke for 45 days caused the increase of ROS in blood content, which is the result of toxic effect on the body. The immature rats were the most sensitive to the affection, which ROS contents in neutrophils increased in 2.75 times till the end of the experiment, in the mature animals it was in 1.65 times higher than in the intacts, in the senile it was higher in 2.43 times than normal rate. It was found that the level of oxygen metabolites and TBA-active products increased during the experiment (on the 15th, 30th and 45th day of toxicity).

Conclusions. The most pronounced changes were inherent for the immature rats. Accumulation of active toxic metabolites was conducted with endogenous intoxication intensifying that was proved by the high content of catabolism products in the body – middle mass molecules that are likely to grow in serum of rats of all age groups.


Keywords


oxidative processes; lipid peroxidation; reactive oxygen species; endogenous intoxication; tobacco smoke; rats.

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DOI: http://dx.doi.org/10.11603/ijmmr.2413-6077.2016.2.7036

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